The solvent supply system is made up of a pump, by which solvent (cell phase) is sent at a managed circulation price. If air receives dissolved in the mobile section, it may make air bubbles that fluctuate the stream fee.
Integrator is the computer-based knowledge processor utilized to file the electronic signal. Simple to specially created software is developed for HPLC.
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- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
are made by reacting the silica particles by having an organochlorosilane of the overall sort Si(CH3)2RCl, exactly where R is really an alkyl or substituted alkyl group.
. The working pump as well as equilibrating pump Every Possess a piston whose forwards and backwards motion maintains a constant circulation rate of around a number of mL/min and supplies the high output force required to drive the cellular period through the chromatographic column.
규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
The elution purchase of solutes in HPLC is ruled by polarity. For a traditional-phase separation, a solute of reduced polarity spends proportionally fewer time within the polar stationary stage and elutes before a solute that is certainly extra polar. Given a particular stationary period, retention periods in ordinary-section HPLC are managed by altering the cellular period’s Houses. One example is, Should the resolution between two solutes is bad, switching to a much less polar cell section retains the solutes over the column for an extended time and supplies far more chance for their separation.
The purchase of elution of compounds with the column is ruled by the intensity of contact with the stationary period. The eluent Along with the separated chemicals flows earlier the detector.
During this specific instrument, Every pump sends its cell stage to a mixing chamber in which they Incorporate to kind the final mobile stage. The relative pace of the two pumps determines the cellular period’s remaining composition.
The mobile stage flows through the stationary phase and carries the factors in the combination with it. Diverse factors travel at distinct premiums. As a result the parts separated and located in numerous location in chromatography to individual, establish and quantify.
From the ionization chamber the remaining molecules—a mixture on the cell phase elements and solutes—bear ionization check here and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-charge ratio (m/z). A detector counts the ions and displays the mass spectrum.
The Exhibit will likely be recorded for a number of peaks- each represents the Every element inside the mixture which often can soak up UV mild. The region of the peak is proportional to the amount of the part handed in the detector.
The smaller check here particles Have a very A great deal higher floor space for interactions in between the stationary period as well as molecules flowing past it. This leads to a far better separation with the factors of the mixture.